The invention discloses an ointment for killing skin wound pyogenic coccus. The ointment comprises the following components in parts by mass: 20-30 parts of frankincense, 20-50 parts of cassia twig, 30-60 parts of Tokyo violet herb and 15-40 parts of alpinia officinarum hance, the above four components are ground to powder, the powder passes through a 500-mesh sieve, mixing the sieved powder is uniformly mixed with 80-150 mass parts of monoglyceride. Compared with the prior art, a sterilization component in the ointment has targeted killing effect for pyogenic coccus, and the ointment is directly smeared at the skin wound, and can avoid the injury on human organ.