The utility model discloses a multifunctional O-shaped rubber sealing ring performance detection device. The device comprises a base, pushing devices, rotary driving devices, pressing plates, a mounting plate and a hanging rod; two sides of the base are respectively provided with the two pushing devices; one rotary driving device is mounted at the inner side of each of the pushing devices; one pressing plate is mounted at the inner side of each of the rotary driving devices; a mounting interval is defined between the pressing plates; the pushing device pushes the rotary driving device to moveto the mounting interval; the rotary driving device controls the pressing plate to perform rotary movement; the mounting plate is installed directly above the mounting interval; the hanging rod is rotatably connected to the bottom of the mounting plate; the bottom of the hanging rod is provided with an elastic opening-closing chuck; and the elastic opening-closing chuck is located directly above the mounting interval. With the multifunctional O-shaped rubber sealing ring performance detection device of the invention adopted, the integrated detection of extrusion performance, friction performance and tensile performance can be realized.