The invention discloses a layered fungus culture rack for an agricultural greenhouse. The layered fungus culture rack comprises a base, shelves, water tanks and butt-joint columns. The shelves are fixedly connected to the upper portion of the base, door frames are hinged to openings of the shelves, steel wire grids are fixedly connected to inner walls of the door frames, and lock noses are fixedlyconnected to outer walls of the door frames and fittingly connected with butt-joint lock fasteners fixedly connected to the surface of the base. The water tanks are fixedly connected to upper ends ofthe shelves, outer walls of the water tanks are provided with water adding holes, and rubber plugs are inserted into the water adding holes. Bottom surfaces of the water tanks are provided with necking water holes, and sponge pads are adhered to inner walls of the necking water holes. The layered fungus culture rack is small in size and can be morphologically changed according to sites to expandan application range. In addition, the layered fungus culture rack is remarkable in transferring performance and conveniently recyclable.