The invention discloses a valve seat ring of an alcohol fuel engine and a manufacturing method thereof, an alcohol fuel engine and an automobile. The inlet valve seat ring of the valve seat ring of the alcohol fuel engine is prepared from the following components in percentage by weight: 0.7-1.3% of C, 0.3-1.0% of Si, 0.1-0.5% of S, 1.0-4.0% of Cr, 5.0-10.0% of Mo, 17.0-21.0% of Co, less than or equal to 0.04% of W, less than or equal to 1.0% of other impurities and the balance Fe, wherein Cu is not included; an exhaust valve seat ring is prepared from the following components in percentage byweight: 0.7-1.3% of C, 0.3-1.0% of Si, less than or equal to 0.04% of S, 3.0-6.0% of Cr, 6.0-9.0% of Mo, 17.0-21.0% of Co, 1.5-3.0% of W, less than or equal to 2.0% of other impurities and the balance Fe, wherein Cu is not included. The valve seat ring disclosed by the invention has a good high temperature mechanical property, good in high temperature attenuating resistance in hardness and good in corrosion resistance of an alcohol fuel.