The invention provides a self-closing valve for perfusion. The self-closing valve comprises a cylinder barrel; the cylinder barrel is internally provided with a piston rod; an annular connector is mounted at one end of the cylinder barrel and the inner diameter of the annular connector is equal to the outer diameter of the piston rod; an annular guide sleeve is mounted at the other end of the cylinder barrel, and the inner diameter of the annular guide sleeve is equal to the outer diameter of the piston rod; a compression spring sleeves the peripheral surface of the cylinder barrel; one end ofthe compression spring abuts against an annular protruding part in the middle of the cylinder barrel and the other end of the compression spring abuts against the annular connector; the piston rod isof an H-shaped structure with a longitudinal section; a plurality of through holes I are formed in a groove wall I of the piston rod close to the annular connector; when the annular protruding part abuts against the annular guide sleeve by the piston rod under the action of the compression spring, the plurality of through holes I are formed in a gap between the cylinder barrel and the piston rod;when the piston rod is compressed, the plurality of through holes I are located in the annular connector; a plurality of through holes II are formed in a groove wall II between a separation part in the piston rod and the annular protruding part. The self-closing valve for perfusion is simple in structure and novel in design and can be automatically closed and opened.