The invention provides an identification method for liver cancer stem cells. According to research finding, LCSC (liver cancer stem cells) can express the unique protective proteins intracellularly orextracellularly under the certain induction condition; based on the beneficial finding, the invention further studies the specificity and the sequence signature of the proteins, and obtains two protein segments which have the good specificity on liver cancer cells and common liver cells through screening, and the test verifies that the proteins are only stably expressed in LCSC while do not existin common liver cancer cells, therefore the proteins can serve as a marker for identifying LCSC. On the basis, the invention optimizes the cell induction method aiming at ensuring the expression quantity and the stability of the protein segments, and by using the method to induce cell samples, not only can the stable expression of the marker protein be ensured, but also the expression quantity and the LCSC cell content present a good linear relation; and based on the rule, relative quantitative analysis of the LCSC cell content can be achieved.