The invention discloses special feed with a function of improving low temperature resistance of soft-shelled turtles and a preparation method of the special feed. The special feed is prepared from cortex cinnamomi, flos caryophylli, fructus galangae, fructus evodiae, rhizoma kaempferiae, semen canavaliae, rhizoma galangae, radix aconiti carmichaeli, microbial flora, rapeseed meal, amino acid powder, bone meal, wheat bran, earthworm powder, sodium bicarbonate, honey, apple juice, attapulgite, chitosan and amylodextrin. The method includes steps: 1) crushing Chinese herbal medicines, adding yellow rice wine, and performing stir-frying; 2) performing inoculation of the microbial flora in the Chinese herbal medicines to realize fermentation, heating and boiling to obtain a cured fermented material; 3) adding water to boil the material, cooling, adding sodium bicarbonate, honey and apple juice, and oscillating to obtain primary pulp of feed; 4) mixing materials obtained in the steps 2) and3), adding attapulgite, chitosan and amylodextrin, granulating and drying to obtain the special feed. The special feed is capable of improving activity of digestive enzymes, promoting digestion, accelerating metabolism, increasing body temperature and improving low temperature resistance of the soft-shelled turtles.