The invention provides an industrial cultivation system for flammulina velutipes. In an industrial cultivation process for flammulina velutipes, a mixing process, a loading process, a sterilization process, a cooling process, an inoculation process, a spawn running process and a fruiting process are all improved. A cultivation control device can acquire and control all process data of cultivation.In the mixing process, the quantity of each raw material and the total quantity can be controlled; the water content is controlled and the stirring degree is controlled; in the loading process, automatic control is realized, the duration of single loading and compacting and the number of times of loading of cultivation bottles can be set and compacting is performed multiple times; in the sterilization process, seamless joint of a loading area and a cooling area is realized, so that the cultivation process is free of disturbance of infectious microbes; the cooling time length and the cooling temperature are strictly controlled; in a digging process, a digging portion and a digging knife are disinfected and automatic operation is realized.