The invention relates to a Lycoris herb genetic transformation system constructing method, and belongs to the technical field of genetics. According to the Lycoris herb genetic transformation system constructing method, a target exogenous gene is introduced into Lycoris herb callus by using a genetic engineering method, and Lycoris herb test-tube seedlings are obtained through differentiation culture and strong seedling culture, such that the new Lycoris herb variety having the target exogenous gene controlled biological traits is obtained. According to the present invention, the method has characteristics of good repeatability and high success rate, wherein the success rate reaches 60-80%; and the establishment of the Lycoris herb genetic transformation system develops the new way for thecultivation of new Lycoris herb varieties.