The invention discloses a method for extracting tigogenin from agave sisalana residue, belongs to the technical field of active component extraction. The method comprises the following steps of pretreatment of raw materials, enzymatic hydrolysis, fermentation treatment, centrifugal collection, purifying treatment and drying treatment. During enzymatic hydrolysis process, the tigogenin is fully separated out by a compound enzyme and the separating rate is quickened. The compound enzyme is prepared from the following raw materials: 18 to 26 parts of cellulase, 10 to 15 parts of pectinase, 1 to3 parts of glucosidase, 2 to 4 parts of proteolytic enzyme and 2 to 4 parts of amylase. During fermentation treatment process, the separating rate of the tigogenin is further improved by active mixedyeast, and the active mixed yeast is prepared from the following raw materials: 6 to 10 parts of aspergillus niger, 3 to 5 parts of neurospora, 4 to 8 parts of penicillium, 6 to 8 parts of trichodermaharzianum, 6 to 10 parts of torulaspora debrueckii and 8 to 12 parts of ethanol-extracted candida. According to the method, the problem that the extracting time is long and the yield of the tigogeninis low in the existing method for extracting tigogenin from agave sisalana residue is solved.