The invention provides a cultivation method using a microbial agent for blocking and preventing tobacco disease infection, and aims at solving the problem that due to damagecaused by soil, extreme climateevents and field operation on tobacco plants, the disease infection rate of the tobacco plants is multiplied. The cultivation method using the microbial agent for blocking and preventing tobacco disease infection includes the following steps of 1, selecting the microbial agent, an application period and an application method, 2, adopting a tobacco planting method, 3, conducting field management, and 4, harvesting tobacco. The cultivation method using the microbial agent for blocking and preventing tobacco disease infection has the advantages that by spraying the microbial agent to leaf surfaces, the tobacco leaf surfaces can be uniformly covered with a layer of beneficial microorganisms, and tobacco disease infection is physically blocked; 2, active substances such as bacterial products and bacterial peptide which are generated by the beneficial microorganisms have significant inhibition effects on tobacco disease infection, and accordingly chemical blocking is achieved; 3, microbial communities in the microbial agent can promote reproduction of beneficial microorganism communities, infection of soil pathogenic microorganisms is inhibited, the resistance and the metabolic function are improved, growth and development of crops are promoted, the soil microenvironment is improved, and source blocking is achieved.