The invention belongs to the technical field of feed processing, and particularly relates to a preparation method of a probiotic fermented chicken feed. The preparation method comprises the followingpreparation steps of (1) weighing raw materials of rice bran and corn flour; (2) taking composite probiotics A, uniformly mixing the taken composite probiotics A with the rice bran to obtain a mixture, adding water, performing sealed fermentation, performing baking, and performing crushing so as to obtain fermented rice bran; (3) dividing the corn flour into two parts, enabling half of the corn flour to be in standby application, uniformly mixing the other part of the corn flour with composite probiotics B, adding water, performing sealed fermentation, performing baking, performing crushing toobtain fermented corn flour, and then uniformly mixing the fermented corn flour with the other half of the corn flour for standby application so as to obtain mixed corn flour; and (4) uniformly mixing the fermented rice bran obtained in the step (2) with the mixed corn flour obtained in the step (3), and performing granulation so as to obtain the probiotic fermented chicken feed. According to thepreparation method disclosed by the invention, the chicken feed is prepared through fermentation with probiotics, and only through simple compounding ratio of the raw materials, the prepared feed canmeet growth requirements of chickens; and besides, chickens fed with the feed are fragrant, sweet and compact in meat quality, and chicken broth is clear, fresh and sweet.