The invention discloses an adsorption layer of a Geminized amphiphilic polymer as well as a preparation method and application of the adsorption layer. The preparation method comprises the step of sequentially modifying the surface of a substrate with amphiphilic polymers with different concentrations by virtue of a dynamic adsorption method, so as to obtain the adsorption layer of the Geminized amphiphilic polymer. The adsorption layer of the Geminized amphiphilic polymer is applied to the preparation of ocean anti-pollution materials. Molecules of the adsorption layer of the Geminized amphiphilic polymer are non-toxic, and the adsorption layer is pollution-free to the environment; and the preparation method is easy in operation, low in cost and environment-friendly and can meet the industrial production requirements.