The invention relates to a preparation method of antibacterial spunlace non-woven fabric, and belongs to the technical field of textiles. Straw and corns are mixed in alkali liquid to be boiled to extract organic components of plant fiber, cellulose, lignin and the like, high-pressure hydro-thermal reaction is conducted, silver nitrate is added to the reaction liquid after plant fiber high-pressure hydro-thermal reaction is conducted, the silver nitrate is absorbed on organic substances by using electrovalent bonds and chelation, citric acid and silver ions are utilized to conduct reaction toallow the silver ions to be restored to nano elemental silver, tetrabutyl titanate is adopted as raw material, nano titanium dioxide is generated through a sol-gel method, multiple hydroxyl groups aregrated to the surface of the nano titanium dioxide under the modification of a sodium stearate hot solution, oxalyl chloride is added to further enhance the viscosity among each molecule in the fabric, the titanium dioxide has better photocatalysis, bacteria and viruses can be effectively decomposed through photocatalysis, the antibacterial capability of the fabric is further enhanced, and the non-woven fabric has broader application prospects.