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发明授权
CN108977582B 一种禽传染性支气管炎病毒的实时荧光定量RT-PCR检测方法 有权

一种禽传染性支气管炎病毒的实时荧光定量RT-PCR检测方法
摘要：
本发明提供一种禽传染性支气管炎病毒的实时荧光定量RT‑PCR检测方法，所使用的引物对和探针组，引物和探针具有序列表SEQ ID NO:1至SEQ ID NO:3的碱基序列。本发明提供一种基于分子生物学的快速检测禽传染性支气管炎病毒的方法，以实现对禽传染性支气管炎病毒进行安全、特异、快速、灵敏、简单、高通量的快速检测，从而弥补现有传统检测技术的不足。并且此方法非常适用于高通量检测，能够同时检测大量样品并快速判定结果，而且使用TaqMan荧光探针，避免传统SYBR Green染色法造成的污染和低特异性。
摘要（英）：

The invention provides a real-time fluorescence quantification RT-PCR detection method of a poultry infectious bronchitis virus. The primer pair and probe sets which are applied in the method have thebase sequences of SEQ ID NO:1 to SEQ ID NO: 3 in the sequence table. The method for rapidly detecting the poultry infectious bronchitis virus based on molecular biology achieves rapid detection of the poultry infectious bronchitis virus safely, distinctively, rapidly, sensitively, simply and in high flux, and accordingly the defects of an existing conventional detection technique is made up. Meanwhile, the method is very applicable to high flux detection, and can simultaneously detect a large amount of samples and rapidly judge the results, in addition, the TaqMan fluorescent probes are applied, and thus pollution and low specificity caused by a traditional SYBR Green dyeing method are avoided.

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