The invention discloses an animal and plant cultivation room. Inside the cultivation room, a feeding mouth is disposed on a side wall of a lower box body near the bottom thereof; the side wall of thelower box body near the top thereof is provided with a water inlet which is connected with a water inlet pipe located inside the box body; an upper box body and the lower box body are equipped with ventilation devices; the upper box body is made of a light penetration material; the lower box body is made of a lightproof material; a temperature controller includes a metal outer shell, an input power line, an output power line, an input conducting plate, an output conducting plate, an inclined slide plate, a conductive rolling ball and a wax block; the right end of the inclined slide plate is connected to the right side wall of the metal shell; and the left end of the inclined slide plate is connected to the left side wall of the metal shell. The animal and plant cultivation room not only provide functions of high-quality lighting, ventilation and diet for an animal experimental body, but also can perform cooling, cleaning and medical treatment on the animal experimental body, and finally ensures the healthy growth of the experimental body.