The invention relates to a method for preparing a biocompatible and durable artificial fish reef, and belongs to the technical field of artificial fish reef preparation. According to the method for preparing the biocompatible and durable artificial fish reef, a discarded steel wire fiber is taken as a raw material and modified to obtain a modified steel wire fiber, the modified steel wire fiber are mixed with other concrete raw materials and film injection is conducted to prepare a fish reef body, a layer of hydroxy calcium phosphate is deposited on the surface of the fish reef body to obtaina modified fish reef body, mosses are grown on the surface of the modified fish reef body, finally, the biocompatible and durable artificial fish reef is prepared, carboxyl, amino and potassium rhodanate are chelated and adhered to the surface of the coarsened steel wire fiber by using the chelating properties of carboxyl, amino and potassium rhodanate to form a compact metal chelating layer, thenthe compact metal chelating layer is soaked with natural amphipathic substance lecithin, thereby avoiding rusting and ageing of the steel wire fiber and improving the durability of the fish reef, thefish reef with the surface deposited with hydroxy calcium phosphate is mixed with the mosses, so that the surface of the fish reef is covered with the mosses, and the biocompatible and durable artificial fish reef has excellent biocompatibility and durability and has a wide application prospect.