The invention relates to a Guyuan jelly, especially to a Guyuan jelly capable of benefiting qi and nourishing blood, and a preparation method thereof. Each 1000 ml of the Guyuan jelly is prepared fromthe following raw materials: 50 to 250 g of donkey-hide gelatin, 20 to 200 g of tremella, 100 to 500 g of Rhizoma Polygonati, 50 to 400 g of wolfberries, 50 to 400 g of red dates and 20 to 150 g of longan aril. According to the invention, based on the combination of traditional Chinese medicine and modern pharmacological research, the raw materials like donkey-hide gelatin, tremella, Rhizoma Polygonati, wolfberries, red dates and longan aril are reasonably combined and realize mutual promotion and cooperation, so the Jin'a Guyuan jelly prepared without accessory materials has coordinated andslippery taste, is capable of benefiting qi and nourishing blood, and is especially applicable to females with deficiency in qi and blood; meanwhile, since sweeteners like granulated sugar are not used, the Guyuan jelly is also applicable to people who need to control sugar intake.