The invention provides a safety tool cabinet with a real-time on-line monitoring function. The safety tool cabinet comprises a cabinet body, a cabinet door and a real-time on-line monitoring system. The real-time on-line monitoring system includes a power supply module, a temperature and humidity control device, an instrument monitoring device and a control module. The instrument monitoring devicecomprises a label and a monitor for monitoring label information. The control module includes a control panel, a display screen and a controller. The control panel is provided with operation buttons;and the input end of the controller is connected to the output end of the control panel, and the output end of the controller is connected to the display screen. According to the safety tool cabinetwith the real-time on-line monitoring function, the temperature and humidity control device is used for monitoring and adjusting the temperature and humidity in the cabinet body to enable instrumentsto be in the required temperature and humidity environment; and the position status of each instrument is monitored with the instrument monitoring device, and the monitoring information, such as whether the instruments are lent or not, whether the instruments are returned or not and whether the instruments are in place or not after the return is displayed on the display screen to remind personnelto return the instruments in time and return the instruments in place.