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发明公开
CN109022415A PDGFRβ单克隆重链抗体杂交瘤细胞及其制备方法和应用 审中-实审

PDGFRβ单克隆重链抗体杂交瘤细胞及其制备方法和应用
摘要：
本发明公开了PDGFRβ单克隆重链抗体杂交瘤细胞及其制备方法和应用。该制备方法包括如下步骤：(1)制备免疫原，所述的免疫原的结构通式为：PDGFRβ胞外区域‑恒定区Fc；(2)利用免疫原免疫HCAb小鼠；(3)对HCAb小鼠进行1～7次加强免疫；(4)制备HCAb小鼠的脾细胞悬液并与小鼠骨髓瘤细胞融合得杂交瘤细胞；(5)鉴定杂交瘤细胞。将该杂交瘤用于PDGFRβ单克隆重链抗体的制备成本低、步骤简便，且抗体性质优异，能与人PDGFRβ的胞外区相结合，并高效抑制或阻断PDGFb与PDGFRβ结合，从而下调或切断相应的信号通路。
摘要（英）：

The invention discloses PDGFRbeta monoclonal heavy chain antibody hybridoma cells, preparation method and application thereof. The preparation method comprises the following steps: (1) preparing an immunogen with the structural formula being PDGFRbeta extracellular region-constant region Fc; (2) immunizing HCAb mice with the immunogen; (3) performing booster immunizations on HCAb mice for one to seven times; (4) preparing spleen cell suspension of HCAb mice and performing cell fusion with mouse myeloma cells to obtain hybridoma cells; (5) identifying the hybridoma cells. The hybridoma can be used for the preparation of the PDGFRbeta monoclonal heavy chain antibody with low cost, simple steps and excellent antibody properties. The antibody can bind to the extracellular domain of human PDGFRbeta and efficiently inhibits or blocks the binding of PDGFb to PDGFRbeta, thereby down-regulating or cutting off the corresponding signaling pathways.

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