The invention discloses a micro one-way valve. Multiple groups of one-way valve devices are arranged at corresponding valve ports; an elastic film is arranged at one end, close to each valve port, ofa holding cavity; a holding cavity clapboard is arranged on the inner wall of each holding cavity, and a contact surface is sealed; an upper holding cavity communicates with a lower holding cavity through a center hole of each holding cavity clapboard; each lower holding cavity communicates with a liquid storage tank through an externally connected passageway; electric conjugation liquid is filledin each liquid storage tank and flows in through the externally connected passageway so as to fully filling each holding cavity; a metal circular ring is embedded into a through hole of each holdingcavity clapboard; steel needles are arranged on the periphery of each metal circular ring; the steel needles and each metal circular ring are connected to a high voltage power supply through wires; anasymmetrical electrode and the electric conjugation liquid jointly form an electric field loop after switching on the corresponding high voltage power supply; electric conjugation liquid of each lower holding cavity produces liquid flowing pressure when flowing to each upper holding cavity; and each elastic film is deformed, contacts the corresponding valve port and tightly presses the corresponding valve port so as to control the corresponding valve port to close. The one-way valve provided by the invention is simple in structure, convenient to install, and suitable for controlling a software robot driven by utilizing a fluid.