The invention relates to a non-intrusive winding temperature measurement method for a forced-oil circulation air-cooled layer-type winding transformer. The method comprises the following steps: connecting a forced-oil circulation air-cooled transformer layer-type winding temperature measurement and control instrument with an oil level thermometer, a current transformer, an environmental thermometer, an air-cooled controller, and an oil pump controller together and collecting corresponding data in real time, and synchronously inputting transformer structural parameters, winding types, and structure size parameters; establishing a heat balance equation between a forced-oil circulation air-cooled layer-type winding transformer winding and transformer oil; and calculating the winding temperature of the forced-oil circulation air-cooled layer-type winding transformer. According to the non-intrusive winding temperature measurement method for the forced-oil circulation air-cooled layer-type winding transformer provided by the invention, for the characteristics of the forced-oil circulation air-cooled layer-type winding transformer, the surface oil temperature of the transformer, the winding current, the cooling mode and the operation of a forced-oil circulation air-cooled cooler are fully considered, so that the measurement of the winding temperature under various working conditions of the transformer is more accurate, and the transformer operation and maintenance personnel can master the running state of the transformer in time.