The invention discloses a detection device capable of rapidly detecting color difference of clothes. The detection device comprises a conveying belt, a plurality of soft positioning plates, a spectralcolour photometer, an infrared emitting device, a plurality of infrared receiving devices, an RFID reader and a device control terminal, wherein the conveying belt is driven by a servo drive mechanism and is used for conveying clothing fabrics to be detected; the soft positioning plates are uniformly installed on the conveying belt, and the soft positioning plates are provided with placing grooves for positioning and placing the clothing fabrics to be detected; and negative pressure cavities are formed in the soft positioning plates, and negative pressure fans for providing negative pressurefor the negative pressure cavities are arranged on the side walls of the soft positioning plates. The detection device does not need manual positioning of the clothing fabrics to be detected, has a stable structure and high detection speed, and can automatically carry out continuous color difference detection on the clothing fabrics to be detected and the clothing fabrics to be detected with different colors, the color difference detection is met, and the requirements of high-efficiency production of the clothes are met.