The embodiment of the invention provides a measurement method for contents of five chemical constituents in northeast bitter herb. The chemical constituents comprise chlorogenic acid, isoquercitrin, galuteolin, luteolin and caffeic acid. The method comprises the steps of (1) building a standard curve of the five chemical constituents; (2) acquiring ultra-high performance liquid chromatography of asample solution; and (3) determining the contents of the five chemical constituents in a sample. The ultra-high performance liquid chromatography is employed, the contents of the five chemical constituents in the northeast bitter herb are simultaneously detected, e method has the characteristics of simplicity, rapidness, high stability, high accuracy and good reproducibility, and a basis is provided for quality control of the northeast bitter herb.