The invention discloses a test method for the anti-interference ability of a line controller. The method comprises: monitoring the serial port communication data in the communication interference environment formed by a mobile terminal, the mobile terminal moving around a line controller to be tested according to the set moving line; Step S2: recording the time information when the serial port communication data of the tested wire controller is abnormal, and the position information of the mobile terminal and the serial port communication data of the tested wire controller. The test method forthe anti-interference ability of the wire controller of the invention has high automation degree and can improve the test efficiency. The invention also provides a test system for the anti-interference ability of a wire controller.