The invention relates to the technical field of tiger lily bud bottle planting equipment, in particular to an automatic mycelium stimulation device. The device comprises a rack, an intermittent bottlepulling assembly, a bottle feeding assembly, a cap opening mechanism, a mycelium stimulation assembly and a bottle discharging conveyor; the intermittent bottle pulling assembly comprises a tray, a groove wheel mechanism, a bottle puller and a bottle puller drive motor; the bottle feeding assembly, the cap opening mechanism, the mycelium stimulation assembly and the bottle discharging conveyor are arranged in the circumferential direction of the tray; the bottle feeding assembly comprises a bottle feeding bracket, a roller, a damping wheel and an elastic damping arm; the cap opening mechanismcomprises an outer curved plate and an inner curved plate; the mycelium stimulation assembly comprises a lifting bracket, a lifting cylinder, a turning shovel, a swinging cylinder, a mycelium stimulation knife, a mycelium stimulation knife drive motor, a mycelium stimulation knife sterilization alcohol lamp, an old mycoderma recovery conveyor and a cultivation bottle limit plate. The automatic mycelium stimulation device can automatically open a bottle cap, thereby reducing the labor intensity and improving the production efficiency, and can also automatically conduct high temperature sterilizing on the mycelium stimulation knife to prevent the infection of hybrid bacterium.