The invention discloses a method for cultivating sterile rice calluses by using rice seeds containing endogenous bacteria. The method comprises the following steps: (1), exposing the rice seeds containing the endogenous bacteria in sunlight; (2), rinsing by using sterile water, and performing blow drying; (3), soaking in an aqueous ethanol solution, taking out, and rinsing by using sterile water;(4), soaking by using a sodium hypochlorite solution, taking out, rinsing by using sterile water, and performing blow drying; (5), putting the seeds obtained in the step (4) into an NB basal medium; (6), performing dark culture at 27-29 DEG C, and transferring the seeds without a bacterial contamination sign to a new NB basal medium once every 3 days; culturing till calluses are grown, picking outthe calluses, putting into the new NB basal medium, and performing dark culture at 27-29 DEG C to obtain the sterile rice calluses. According to the method, the sterile calluses are obtained from therice seeds containing the endogenous bacteria; the cost is low; the operation is simple; in addition, the seeds in the breeding process are saved, the cost is low, the operation is simple, and a precious germplasm resource can be cultivated in a rescuing manner.