The invention discloses a novel fruit and vegetable fresh-keeping film and a preparation method thereof. The novel fruit and vegetable fresh-keeping film is prepared from the following raw material components: polyethylene, polyethylene glycol, glycerinum, dodecyl alcohol ester, herba andrographitis extract liquid, radix sophorae flavescentis extract liquid, herba portulacae extract liquid, and functional filler. The preparation method comprises the following steps: (1) mixing the polyethylene, polyethylene glycol, glycerinum and dodecyl alcohol ester, emulsifying and stirring at a high speed,and forming a film matrix; (2) mixing herba andrographitis extract liquid, radix sophorae flavescentis extract liquid and herba portulacae extract liquid, adding the functional filler, heating, stirring, drying, and obtaining powder; (3) adding the powder in step (2) into the film matrix in step (1), stirring, heating, preserving the heat, cooling to room temperature, pouring into a two-screw extruder, plasticating, film blowing, drying, and obtaining the fruit and vegetable fresh-keeping film. The fruit and vegetable fresh-keeping film prepared in the invention is simple in preparation process, low in cost, capable of effectively improving the fresh-keeping quality of fruits and vegetables and prolonging the fresh period of the fruits and vegetables, and safe, environment-friendly and nontoxic.