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发明公开
CN109055310A 一种基于TWS119的NK细胞培养方法 无效 - 撤回

一种基于TWS119的NK细胞培养方法
摘要：
本发明提供了一种基于TWS119的NK细胞培养方法，包括：取静脉血，制备混合补体；对所述混合补体进行分离，得到所述混合补体中的单个核细胞；取单个核细胞与TWS119进行共培养，得到共培养液；对所述共培养液进行细胞重悬，并加入TWS119进行二次培养，即得到NK细胞。本发明在培养过程中，加入了TWS119进行诱导，提高了NK细胞的增殖率和纯度。
摘要（英）：

The invention provides a TWS119 based NK cell culture method which comprises the following steps: taking venous blood and preparing a mixed complement; separating the mixed complement to obtain mononuclear cells in the mixed complement; co-culturing the mononuclear cells and TWS119 to obtain co-culture liquid; performing cell resuspension on the co-culture liquid, adding TWS119 and performing secondary culture to obtain NK cells. According to the method disclosed by the invention, TWS119 is added for induction in the culture process, and the reproduction rate and the purity of the NK cells areincreased.

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