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CN109055614A 基于RAA荧光法检测乙型肝炎病毒的引物探针组及试剂盒 无效 - 撤回

基于RAA荧光法检测乙型肝炎病毒的引物探针组及试剂盒
摘要：
本发明提供了基于RAA荧光法检测乙型肝炎病毒的引物探针组，属于分子生物学检测技术领域，所述引物探针组包括上游引物、下游引物和探针，所述上游引物的核苷酸序列如SEQ ID NO.1所示，所述下游引物的核苷酸序列如SEQ ID NO.2所示，所述探针的核苷酸序列如SEQ ID NO.3所示；所述探针为荧光报告基团和荧光猝灭基团修饰的探针。本发明提供的引物探针组只需15min内即可完成乙型肝炎病毒DNA的检测，无需通过高温使DNA解旋，只需在30～42℃下进行等温扩增即可完成检测，检测快速、灵敏，特异性好，无假阳性。
摘要（英）：

The invention provides a primer probe group for detecting hepatitis B virus based on RAA fluorescence method, and belongs to the technical field of molecular biological detection. The primer probe group comprises an upstream primer, a downstream primer and a probe, wherein the nucleotide sequence of the upstream primer is shown by SEQ ID NO.1, the nucleotide sequence of the downstream primer is shown by SEQ ID NO.2, and the nucleotide sequence of the probe is shown by SEQ ID NO.3; the probe is a modified by a fluorescence reporter group and a fluorescence quenching group. By adopting the primer probe group provided by the invention, detection of the hepatitis B virus DNA can be finished within 15min, high temperature is not required for DNA unwinding, and the detection can be finished simply by performing isothermal amplification at 30-42 DEG C; moreover, the detection is fast and sensitive and has high specificity while avoiding false positive.

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