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发明公开
CN109055615A 一种检测A、B、J和K亚群禽白血病病毒的多重PCR引物组、试剂盒及方法 有权

一种检测A、B、J和K亚群禽白血病病毒的多重PCR引物组、试剂盒及方法
摘要：
本发明公开了一种检测A、B、J和K亚群禽白血病病毒的多重PCR引物组，包括检测禽白血病病毒的通用上游引物SEQ ID NO.1，A亚群禽白血病病毒的下游引物SEQ ID NO.2，B亚群禽白血病病毒的下游引物SEQ ID NO.3，J亚群禽白血病病毒的下游引物SEQ ID NO.4，K亚群禽白血病病毒的下游引物SEQ ID NO.5。本发明还公开了一种检测A、B、J和K亚群禽白血病病毒的多重PCR试剂盒，包括本发明所述的多重PCR引物组、Premix Ex Taq DNA聚合酶、无菌双蒸水、阴阳性对照质粒DNA模板。此外，本发明还公开了一种检测A、B、J和K亚群禽白血病病毒的多重PCR方法。实验证明，应用本发明可以同时从样品中检测出A、B、J和K亚群禽白血病病毒，特异性强，灵敏度高，操作和结果判定简便，适合进行批量样品的检测及外源性ALV的初步鉴定。
摘要（英）：

The invention discloses a multi-PCR (Polymerase Chain Reaction) primer group for detecting A, B, J and K subgroups avian leukosis viruses (ALV). The multi-PCR primer group comprises a common upstreamprimer SEQ ID NO. 1 for detecting the avian leukosis virus, a downstream primer SEQ ID NO. 2 of the A subgroup avian leukosis virus, a downstream primer SEQ ID NO. 3 of the B subgroup avian leukosis virus, a downstream primer SEQ ID NO. 4 of the K subgroup avian leukosis virus and a downstream primer SEQ ID NO. 5 of the K subgroup avian leukosis virus. The invention further discloses a multi-PCR kit for detecting the A, B, J and K subgroups avian leukosis viruses; the multi-PCR kit comprises the multi-PCR primer group provided by the invention, Premix Ex Taq DNA (Deoxyribonucleic Acid) polymerase, sterile double distilled water and a negative and positive control plasmid DNA template. Furthermore, the invention further discloses a multi-PCR method for detecting the A, B, J and K subgroupsavian leukosis viruses. An experiment shows that the multi-PCR primer group provided by the invention can be used for detecting the A, B, J and K subgroups avian leukosis viruses from a sample at thesame time, has the advantages of strong specificity, high sensitivity and convenience for operation and result judgment and is suitable for detecting batch samples and primary identification of exogenous ALV.

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