The invention belongs to the technical field of drug residue detection, and particularly relates to a method for detecting a residual quantity of azithromycin in animal hair. According to the method,the animal hair is taken as a detection object, and livestock such as a chicken, a pig, cattle or the like is not required to be slaughtered, so that a living body of the livestock can be detected; ahair sample is more convenient to obtain than the muscle, the liver, the excrement or the like, and the hair has a long growth period, so that whether the azithromycin is used in the growth process ofthe livestock or not can be accurately monitored; and therefore, the purpose of safety production and supervision of foods such as the livestock and the like is achieved, a detection result is accurate, the situations of false positive, false negative and the like do not occur, and the sensitivity is high.