The invention discloses an internal and external network isolation system based on a virtual isolation technology, which comprises an external network firewall, a wireless network controller, a POE switch, a wireless switch, an internal network firewall, a core switch and a VPN remote office system. The invention also discloses an implementation method of an internal and external network isolationsystem based on a virtual isolation technology. The invention has the beneficial effect that, from a user experience point of view and the difficulties in renovating old office buildings, customer investment can be minimized in terms of input costs, at the same time, it can solve the problem that the physical internal and external network isolation technology can not use the remote office systemthrough the Internet and log in to the internal network to realize the remote office while the security expected goal of the network physical isolation is basically achieved.