The invention relates to an air purifying compound biological enzyme. The air purifying compound biological enzyme is a mixture composed of biological enzymes, protease, glucose oxidase, ligninase, lipase and filler. As a full-spectrum optimal enzyme combined for public places such as newly decorated offices, schools and houses, furniture, hotels, restaurants and malls as well as air purificationand deodorization in different environments such as kitchens, toilets, bathrooms and plant workshops, the air purifying compound biological enzyme can play a role when multiple sources of pollution exist and meanwhile is high in biological activity; meanwhile, by degrading odors and inhibiting odor transformation through a biological enzyme catalytic degrading function, the air purifying compoundbiological enzyme can achieve the aim of deodorization. The invention also discloses a preparation method of the air purifying compound biological enzyme.