The invention relates to the technical field of foods, in particular to an infant formula containing choline chloride and L-carnitine and a preparation method thereof. The infant formula comprises thefollowing raw materials in parts by weight: 20 to 35 parts of whole fat goat milk powder, 25 to 55 parts of desalted whey powder, 0 to 20 parts of concentrated whey protein powder, 0.4 to 10 parts oflactose, 3 to 15 parts of mixed vegetable oil, 5 to 10 parts of 1,3-dioleic acid-2-palmitic acid triglyceride, 2 to 4 parts of galactooligosaccharide, 0.5 to 1 part of fructo-oligose, 0.1 to 0.5 partof calcium carbonate, 0.01 to 0.1 part of choline chloride, 0.2 to 1 part of compound minerals, 0.4 to 0.8 part of compound vitamins, 0.4 to 0.8 part of arachidonic acid (ARA) powder, and 0.1 to 1 part of docosahexaenoic acid (DHA) powder, and is prepared by a certain preparation method. The infant formula containing the choline chloride and the L-carnitine satisfies the nutritional needs of thegrowth and development of normal infants and young children, enhances the absorption and digestive functions of infants and young children, and appropriately enhances the mental development of infantsand young children, and is particularly important for maintaining normal metabolism of premature infants whose biochemical functions are still incomplete.