Disclosed is a using method of medical glass oral liquid bottle comprising a liquid bottle and a pipette, wherein the liquid bottle comprises a bottle cap and a partition plate, the partition plate isprovided with a liquid medicine through hole, and the liquid bottle is divided into a plurality of containers by the partition plate, and the volume of each container is the same. The pipette is provided with a plurality of marks, and the lower pipe opening of the pipette is provided with a liquid suction hole. Each time the adult takes the liquid medicine, the pipette is inserted into the container of the liquid bottle through the liquid pipette groove of the bottle cap, and the liquid medicine of all the containers in the liquid bottle is sucked and taken by the pipette. A liquid pipette isinsert into each container of a liquid bottle through a liquid pipette groove of that bottle cap when the liquid is taken by children, and the liquid in each contain is successively sucked by the liquid pipette, and the liquid in one container of the liquid bottle is taken by children at a time, thereby solving the problem of dosage control of oral liquid taken by children.