The invention relates to the field of medical transportation, in particular to a stem cell injection transportation and preservation device. The stem cell injection transportation and preservation device comprises a transportation cabinet body, a nitrogen spraying device, a liquid nitrogen tank and a gas storage tank, wherein the nitrogen spraying device is installed on the back side of the transportation cabinet body, the liquid nitrogen tank and the gas storage tank are arranged on the left side and the right side of the transportation cabinet body correspondingly, multiple preservation assemblies are arranged in the transportation cabinet body, a control panel is arranged on the outer side of the transportation cabinet body, the liquid nitrogen tank communicates with the nitrogen spraying device, each preservation assembly communicates with the liquid nitrogen tank and the gas storage tank correspondingly, a pressure exhaust device is arranged at the top end of the transportation cabinet body, the pressure exhaust device communicates with the transportation cabinet body and the gas storage tank correspondingly, the nitrogen spraying device can be installed in the transportationcabinet body in a vertical and translational moving mode through a power device, and a pressure detector and a temperature detector are installed in the transportation cabinet body, and are both electrically connected with the control panel. According to the stem cell injection transportation and preservation device, multiple layers of stem cell injection can undergo cold storage treatment layer by layer, then the temperature of a cold storage space can be effectively guaranteed in cooperation with a cold guide pipe and other measures, and then the stability of all the stem cell injection is effectively guaranteed in the transportation process.