The invention discloses functional fabric containing deep sea fish oil and iridium and a preparation method of the functional fabric. The preparation method comprises the following steps: fabric is formed by interweaving iridium-containing nylon yarn and graphene-containing polyester yarn; the fabric is placed in a textile auxiliary agent containing the deep sea fish oil to be soaked at room temperature and dried. The functional fabric combines the iridium-containing nylon yarn with the graphene-containing polyester yarn and is treated by the textile auxiliary agent containing the deep sea fish oil, so that cells are endowed with more energy, cell viability is enhanced, blood flow speed is increased, oxygen carrying capacity and nutrition capacity are improved, and nutrition and viabilityof cells at tail end of the organism are improved.