The invention relates to a method of measuring residual quantity of amitraz and three metabolites, in particular to a method of measuring residual quantity of amitraz and metabolites in honey by meansof a QuEChERS-liquid chromatogram-mass spectrum/mass spectrum method. The method comprises the following steps: adding a buffer solution, the pH of which is 7, into a test sample; carrying out extraction and salting out with acetonitrile and sodium chloride; carrying out dispersive solid-phase extraction on an adsorbent containing N-primary secondary amine (PSA), anhydrous magnesium sulfate and C18 powder to purify an extraction solution; carrying out detection by a liquid chromatogram-mass spectrum/mass spectrometer; and carrying out quantification by means of a isotope interior label diluted internal standard method. The method is green and environmentally friendly, simple and rapid, small in consumed resource and low in detection cost, and the sensitivity of the method can meet the detection and confirmation requirements on the residual quantity of amitraz and the three metabolites in honey.