The present invention discloses a loess highfill aquitard detection method. The method comprises the steps of: the step 1: arranging a high-density measuring wires; the step 2: obtaining specific resistance data; the step 3: obtaining a specific resistance profile map; the step 4: obtaining the quantity and the distribution of an undetermined layer of the aquitard on the area section to be detected of the loess highfill; the step 5: constructing a loess highfill three-dimensional view; and the step 6: performing measurement of the aquitard. The loess highfill aquitard detection method employsthe high-density electrical method to detect the loess highfill area to obtain the quantity and the distribution of the undetermined layer of the aquitard on the area section to be detected of the loess highfill and achieve the rough detection of the aquitard of the loess highfill area, and employs a geological drill to perform sampling and measure the moisture content of the undetermined layer ofthe aquitard so as to achieve the accurate detection of the aquitard of the loess highfill area, reduce the input cost and perform full coverage and greatly improve the working efficiency.