The invention relates to a gastroscope sampling device, which comprises a main body part, a manifold block, a sampling device and a setting-out device. The manifold block is arranged on that main bodypart. The sampling device is disposed on the main body part and comprises a first accommodating groove, a moving body, a rotating lever, a first connecting rod and a sampling assembly, wherein the moving body is arranged in the first accommodating groove and the moving body is slidable along the first accommodating groove, and the rotating lever, the first connecting rod and the sampling assemblyare sequentially connected. A lofting device comprises a second connecting rod and a supporting rod, wherein the supporting rod is provided with a lofting groove, the lofting groove is provided witha door body capable of opening and closing, the door body is connected with the second connecting rod, the second connecting rod is also hinged with the rotating rod, and the rotating rod is also hinged with the supporting rod. The gastroscope sampling device of the invention does not cause discomfort to human body, has no limitation on the applicable object, and can be used for omnidirectional inspection.