The invention belongs to the technical field of plant cultivation, and particularly discloses a cultivation method for pleurotus djamor. The method comprises the following steps of material preparation, fermentation, bagging, inoculation, soil laying, cultivation soil sterilization, pit digging, bag removal, soil covering, fruiting and harvesting. According to the scheme, a cultivation device is adopted and comprises a cultivation groove, a liquid groove, a plurality of partition plates and a plurality of treatment mechanisms, the partition plates are fixedly arranged in the cultivation grooveand define a plurality of planting pits, the liquid groove is formed in the bottom of the cultivation groove and provided with a liquid inlet, and a heater is arranged in the liquid groove; each treatment mechanism comprises a through groove, a vertical cylinder, two sieve plates and two elastic plates, the through grooves are formed in the bottom of the cultivation groove, the cross section of each vertical cylinder is square, the vertical cylinders are vertically arranged on the side walls of the through grooves, one end of each vertical cylinder stretches into the liquid groove, and everytwo sieve plates are hinged to each other. The cultivation method solves the problem that the early pre-treatment process of soil required in the cultivation process of pleurotus djamor is complicated.