The invention provides a display brightness compensation method and device and equipment. The display brightness compensation method comprises the steps that brightness of each pixel block in a targetarea is acquired; the initial reference brightness is adjusted according to the brightness of each pixel block, and target compensation data of each pixel block are calculated according to the adjusted reference brightness and the brightness of each pixel block, wherein the target compensation data are within a predetermined compensation range; and according to the target compensation data of each pixel block, the brightness of each pixel block is compensated. According to the display brightness compensation method and device and the equipment, on the premise of ensuring compensation precision, the compensation effect of uneven brightness is improved, and the display performance of a display screen is improved.