The invention discloses an automatic preparation method and device of tobacco pollen. The device disclosed by the invention is composed of an anther separation module, an anther sieving module, an anther drying module and a pollen collection module; when the device is used, procedures including tobacco anther threshing, impurity removal, anther drying, pollen sieving, pollen collection and the like can be automatically carried out according to the sequence; finally, automatic preparation of the tobacco pollen is finished. The method has the advantages of less manual operation, high automationdegree, good product quality and the like. Compared with traditional manual pollen preparation, the labor cost can be extremely saved; the drying time can be shortened by 60 percent in a drying and sieving link; sunlight ultraviolet irradiation is isolated in a drying link, so that damage to the pollen is reduced and the average pollen vitality is improved by 20 percent or more.