The embodiment of the invention provides a display panel, a fabrication method thereof and a display device, which relate to the technical field of display and can solve the problem of low color gamutof the display panel. The display panel comprises multiple sub-pixels, a substrate and pixel circuits, wherein each pixel circuit is located on the substrate and arranged in each sub-pixel; and the pixel circuit comprises a TFT. The display panel also comprises a pixel definition layer covering the TFT, wherein the pixel definition layer comprises multiple grooves and isolation columns located around the grooves; the positive projection of the groove on the substrate is located in a light emitting area of the sub-pixel; and the groove is internally provided with a quantum dot light emitting diode electrically connected with the above TFT. The display panel is used for display.