The invention discloses a preparation method of a super-hydrophobic surface with a directional transport function. The method comprises the following steps: step 1, spin coating a first layer of ultraviolet lithography positive photoresist for the first ultraviolet lithography; step 2, spin coating a second layer of ultraviolet lithography negative photoresist, and tilting a substrate for the second ultraviolet lithography; step 3, dip coating low surface energy nanoparticles; and step 4, evaporating a hydrophilic metal thin film in a vacuum mode, and generating a required directional transport track to obtain the super-hydrophobic surface with the directional transport function. According to the preparation method of the super-hydrophobic surface with the directional transport function provided by the invention, through twice ultraviolet lithography, the specific surface area of a microstructure is increased on one hand, so that the roughness after dip coating the low-surface energy nanoparticles is sufficient; and on the other hand, when the second lithography is performed, the substrate is intentionally inclined for exposure, so that a one-way inclined structure with a certain inclination angle is obtained, therefore a rolling direction is given in advance. The pre-given rolling direction is combined with a hydrophilic transportation track obtained through later evaporation,so that the directional transport of liquid drops is better realized.