The invention discloses a tissue culture method for robinia pseudoacacia. According to the method, MS basic medium is improved, a large quantity of elements in a GS medium are adopted, meanwhile, a large quantity of elements of MS are removed, concentration and ratio of inorganic salts in the MS medium are improved, the adventitious bud induction rate can be effectively increased after the basic medium is formed by combination, and furthermore, cultured tissue culture seedlings have high survival rate, grow vigorously after culture and have strong growth potential.