The invention discloses an ovary upkeeping composition containing NADH and DHEA. The ovary upkeeping composition containing NADH and DHEA is prepared from main materials and auxiliary materials. The main materials include the following components in parts by weight: 1-10 parts of DADH, 1-8 parts of DHEA, 2-9 parts of deglycosylated soy isoflavone, 1-10 parts of sheep placenta extract, 3-8 parts ofsialic acid, 2-7 parts of s-ademetionine and 1-10 parts of herba epimedii secondary aglycone. The combination of effective components of DADH, DHEA and the like with traditional Chinese medicine components is first proposed, theories of traditional Chinese medicine and western medicine work in concert to generate synergistic interaction action. The composition is reasonable in formula and properin combination and has an excellent ovary upkeeping effect. The invention also discloses a preparation method containing the upkeeping composition. The main materials and the pure traditional Chinesemedicine auxiliary materials are matched and coordinate, so that the composition has the functions of adjusting internal secretion of human body, adjusting and balancing hormones, increasing blood manufacturing, improving anemia, qi deficiency, enhancing cell vitality and the like. The preparation is simple in preparation process, mild in condition and suitable for industrial quantity production.