The invention relates to a fixing tool for tempered glass manufacturing, production and pretreatment, and a fixing method of the fixing tool. The fixing tool comprises a bottom plate, a rotating motoris mounted on the bottom plate through a motor seat, a rotating storing plate is mounted on an output shaft of the rotating motor, a hoisting frame is mounted on the bottom plate, a lifting telescopic air cylinder is mounted in the middle of the hoisting frame, the top end of the lifting telescopic air cylinder is mounted on the lifting frame through a flange, and a lifting telescopic rod is mounted between the lifting frame and the hoisting frame. According to the fixing tool, the problems that in the existing glass production, manufacturing, pretreatment and processing processes, glass needs to be manually fixed by an existing clamping tool and then subjected to processing operation of multiple working procedures such as cutting, edge breaking-off and drilling, an existing clamp is simple in structure, adjusting is troublesome when the glass of different specifications is fixed, position fixing is inaccurate, the glass is prone to shaking due to external force during processing, andconsequently, the stability of the glass during processing cannot be ensured can be solved.