The invention relates to the technical field of cotton yarn textile and particularly relates to an anti-mildew pure cotton yarn textile technology. The anti-mildew pure cotton yarn textile technologycomprises a blowing-carding process, a combing process, a drawing process, a roving process, a spinning process, a spooling process and a packaging process; after the roving process and before the spinning process, roving is immersed into alkali solution, then is immersed into weak acid solution after being taken out to be dried and washed, then is taken out to be dried and washed again, is soakedwith an antibacterial solvent and then is subjected to ultrasonic treatment, the treated roving is subjected to centrifugal dewatering and transferred into a sealed cabin, and after a 254 nm ultraviolet ray is used for irradiating the roving to sterilize, the subsequent spinning step is carried out; the antibacterial solvent is prepared from 8 to 13 parts of melamine, 5 to 8 parts of sodium polyacrylate, 1 to 3 parts of glycerinum, 15 to 20 parts of polyvinyl alcohol, 1 to 3 parts of starch, 10 to 18 parts of nano-silica and 40 to 50 parts of water. According to the invention, the acid and alkali solution and the antibacterial solvent are adopted to carry out sterilization and modification on raw materials, so that an antibacterial effect of finished product yarns can be improved, and mildew is prevented.